NMR

The Nuclear Magnetic Resonance (NMR) Core was established in 2016. The NMR Core at iHuman mainly focuses on life science and chemistry, especially on: the three-dimensional structure of biomacromolecules in solution; the relationship between structure, function and kinetics of biomacromolecules in solution; the interaction between biomacromolecules; and the interaction between biomacromolecules and small molecules. It includes two state-of-the-art Bruker NMR instruments:

1.     The 800 MHz NMR is equipped with room temperature 5 mm and 1.7 mm ¹H-¹³C-¹⁵N inverse probes with high resolution and S/N. This gives iHuman the ability to conduct NMR tests of proteins from one dimension to multi-dimension, non-uniform sampling, chemical structure identification and drug screening.

2.     The 600 MHz NMR is equipped with a 5 mm ¹H/¹⁹F-¹³C-¹⁵N inverse cryo-probe (optimized for 19F detection) and a 5 mm ¹H-¹⁹F-¹³C-¹⁵N quadruple resonance room temperature probe. The S/N of cryo-probe is more than 7700 and 7300 for ¹H and ¹⁹F, respectively. Except for the protein NMR and chemical tests which are similar to 800M, the 600 MHz NMR offers ¹⁹F detection especially on fluorine-labeled proteins and ligands. These can be used for detecting structural changes at specific sites of protein or protein-ligand interactions on the GPCR family. This technology has already been used successfully for iHuman’s Glp1, GCGR and CB1 projects.