The Bio-EM Facility of ShanghaiTech University (https://bioem.shanghaitech.edu.cn) provides critical cryo-electron microscopy (cryo-EM) infrastructure and GPU-based computing resources to support scientific research, particularly in the life sciences. Cryo-EM enables the determination of high-resolution three-dimensional structures of biological macromolecules and subcellular assemblies across multiple levels of structural organization. The facility is dedicated to supporting research in structural biology, cell biology, and related translational fields. Its primary technical capabilities include cryo-EM single-particle analysis (cryo-EM SPA), cryo-electron tomography (cryo-ET), and electron microscopy studies of cells and tissues.
Currently, the facility houses six cryo-transmission electron microscopes, including three 300kV TEMs, one 200kV TEM, and two 120kV TEMs, as well as two cryo-focused ion beam scanning electron microscopes (cryo-FIB-SEM). The facility is also equipped with a comprehensive suite of sample preparation instruments, including a cryo-confocal microscope, a high-pressure freezer, a cryo-ultramicrotome, and plunge freezers.
In addition, the facility operates a high-performance computing cluster for image data storage, processing, and reconstruction. Together, these resources enable the facility to provide integrated services covering the full cryo-EM workflow.
