50年来，位于瑞士苏黎世联邦理工学院（ETH Zürich）、美国加利福尼亚州拉霍亚斯克里普斯研究所（Scripps Research）以及中国上海科技大学iHuman研究所的Wüthrich研究组一直致力于生物大分子核磁共振光谱学（NMR）的研究。其研究成果包括利用溶液核磁共振（NMR）测定大分子结构的方法，以及将横向弛豫优化光谱（TROSY）原理应用于大型超分子组装体的NMR实验。多年来，相关应用主要集中于高等生物的分化、免疫抑制和神经病理学领域。截至到2026年6月1日，Wüthrich研究组在上海科技大学继续开展基于NMR技术的研究，重点关注G蛋白偶联受体（GPCRs）。

Journal of the American Chemical Society 2025 147(48):44114-44122

Cell Chemical Biology 2025 32(9):1140-1149.e3.

Ge, H., Yang, L., Chen, C., Xu, Y., Liu, D. and Wüthrich, K. (2025) J. Am. Chem. Soc. 147, 44114–44122. Human Substance P Interactions with G Protein-Coupled Receptor NK1R Observed by NMR in Solution.

Guo, C., Yang, L., Liu, J., Liu, D. and Wüthrich, K. (2025) Cell Chem. Biol. 32, 1140–1149. Structural basis of adenosine 2A receptor-balanced signaling activation relies on allosterically mediated structural dynamics.

Pan, B., Liu, D., Yang, L. and Wüthrich, K. (2022) Proc. Natl. Acad. Sci. USA 119, doi: 10.1073/pnas.2122682119. GPCR large-amplitude dynamics by ¹⁹F-NMR of aprepitant bound to the neurokinin 1 receptor.

Shimada, I., Ueda, T., Kofuku, Y., Eddy, M.T. and Wüthrich, K. (2019) Nat. Rev. Drug Disc. 18, 59–82. GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures.

Eddy, M.T., Lee, M.-Y., Gao, Z.-G., White, K.L., Didenko, T., Horst, R., Audet, M., Stanczak, P., McClary, K.M., Han, G.W., Jacobson, K.A., Stevens, R. and Wüthrich, K. (2018) Cell 172, 68–80. Allosteric coupling of drug binding and intracellular signaling in the A_2A adenosine receptor.

Liu, J.J., Horst, R., Katritch, V., Stevens, R.C. and Wüthrich, K. (2012) Science 335, 1106–1110. Biased signaling pathways in β₂-adrenergic receptor characterized by ¹⁹F-NMR.

Wüthrich, K. (2003) Angew.Chem. Int. Ed. 42, 3340−3363. NMR studies of structure and function of biological macromolecules (Nobel Lecture).

Fiaux, J., Bertelsen, E., Horwich, A. and Wüthrich, K. (2002) Nature 418, 207–211. NMR analysis of a 900K GroEL–GroES complex.

Pervushin, K., Riek, R., Wider, G. and Wüthrich, K. (1997) Proc. Natl. Acad. Sci. USA 94, 12366–12371. Attenuated T₂ relaxation by mutual cancellation of dipole–dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

Wüthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley, New York.

Wüthrich, K. (1969) Proc. Natl. Acad. Sci. USA 63, 1071–1078. High resolution proton nuclear magnetic resonance spectroscopy of cytochrome c.

Wüthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley, New York.

Wüthrich, K. (2021) NMR with Biological Macromolecules in Solution, World Scientific, Singapore.

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