The Assay Development Core is currently focused on establishing functional assays as well as searching for novel small molecule ligands of human G protein coupled receptors(GPCRs).
GPCRs, also known as seven transmembrane domain receptors, are the largest and most diverse family of membrane receptors in eukaryotes. Over 40% of approved drugs on market target GPCRs, and therefore finding novel ligands for GPCRs remains a hot therapeutic topic.
Protein based assay
CPM: test protein thermostability
SPR: KD measurement
Cell based assay
cAMP: Gi/Gs coupled GPCR
FLIPR Ca2+ : Gq, could be universal
Tango: β-arrestin recruitment
Other reporter gene assay