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Assay Development Core

[source]:超级管理员 [the author]:超级管理员 [date]:2018-09-14 [Views]:336

The Assay Development Core is currently focused on establishing functional assays as well as searching for novel small molecule ligands of human G protein coupled receptors(GPCRs).

 GPCRs, also known as seven transmembrane domain receptors, are the largest and most diverse family of membrane receptors in eukaryotes. Over 40% of approved drugs on market target GPCRs, and therefore finding novel ligands for GPCRs remains a hot therapeutic topic.

Protein based assay

CPM: test protein thermostability 

SPR: KD measurement

Cell based assay

cAMP: Gi/Gs coupled GPCR 

FLIPR Ca2+ : Gq, could be universal 

Tango: β-arrestin recruitment 

Other reporter gene assay 

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