Qingtao Shen Research Associate Professor


InstituteiHuman Institute
Research AreaOn interplays between protein and membrane using cryo-EM/ET

Dr. Shen got his bachelor’s degree in 2001 and master’s degree in2004 at School of Life Science, Shandong University, and then obtained his Ph.D degree at School of Life Science, Tsinghua University in 2009. From 2009 to 2016, Dr. Shen received his postdoctoral training in the field of cryo-electron microscopy/tomography at Yale University, University of Wisconsin-Madison and University of California-Berkeley. In July 2016, Dr. Shen joined ShanghaiTech University dually appointed as an assistant professor at School of Life Science and Technology and as a research associate professor (PI) at iHuman institute.
helix2Research Interests
Cells especially eukaryotic cells have evolved complicated intracellular membrane system. The interplay between proteins and membrane lipids is central to almost every aspects of cell biology. These interactions not only determine cell and organelle shape and the evolution of shape over time, but also turn on and off the signals that control essential cell processes and communication between cells. Meanwhile, pathogens such as HIV-1 also subvert and co-opt these interactions to invade the hosts. My research interest is to reveal the structure of membrane-interacting proteins, leading to molecular mechanism of protein-membrane interactions.
Cryo-Electron Microscopy/Electron Tomography (cryo-EM/ET) has already been proven powerful to examine both in vivo and in vitro heterogeneous samples at diverse scales from cell section to individual proteins with high resolution. These merits draw cryo-EM/ET the heart of my tools, complemented with biochemistry, fluorescence imaging, x-ray crystallography and dynamic simulation, which will lead to a constant stream of new hypotheses about biological function and mechanism. I will also place the highest value upon making direct tests of these hypotheses in cells, and this is the integral map of my research.
bookSelected Publications

1. Lars-Anders Carlson, Qing-Tao Shen, Mark Remec Pavlin, James H. Hurley (2015) ESCRT filaments as spiral springs. Developmental Cell, 35(4): 397-398

2. Qing-Tao Shen, Xuefeng Ren, Rui Zhang, II-Hyung Lee, James H. Hurley (2015) HIV-1 Nef hijacks clathrin coats by stabilizing AP-1:Arf1 polygons. Science, 350(6259): aac5137 (Faculty 1000 recommended)

3. Bei Yang, Goran Stjepanovic, Qing-Tao Shen, Andreas Martin, James H. Hurley (2015) The AAA+ ATPase dissembles ESCRT-III by global unfolding and pore translocation. Nature Structural & Molecular Biology, 22(6): 492-498

4. Qing-Tao Shen, Amber L. Schuh, Yuqing Zheng, Marisa S Otegui, Paul Alquist, Qiang Cui, Anjon Audhya (2014) Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly. Journal of Cell Biology (Cover Story), 206(6):763-777 (Highlighted in Journal of Cell Biology 206(5)) (Faculty 1000 recommended)

5. Zi-Chao Yu, Xiu-Lan Chen, Qing-Tao Shen, Dian-Li Zhao, Bai-Lu Tang, Hai-Nan Su, Zhao-Yu Wu, Qi-Long Zhao, Bin-Bin Xie, Xiying Zhang, Yong Wu, Bai-Cheng Zhou, Bo Chen, Yu-Zhong Zhang (2014) Filamentous phages prevalent in Psuedoalteromonas spp. confer properties advantageous to host survival in Arctic sea ice. ISME Journal, 1-11

6. Lei Shi, Kevin Howan, Qing-Tao Shen, Yong Jian Wang, James E Rothman, Frederic Pincet (2013) Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores. Nature Protocols, 8:935-948

7. Qing-Tao Shen, Peter Hsiue, Charles V. Sindeler, Matthew D. Weltch, Ken G. Campellone, Hong-Wei Wang (2012) Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling. Journal of Cell Biology (Cover Story), 199(1): 111-124(In focus by Journal of Cell Biology 199(1))

8. Lei Shi, Qing-Tao Shen, Alexander Kiel, Jing Wang, Hong-Wei Wang, Thomas J. Melia, James E. Rothman, Frederic Pincet (2012) SNARE Proteins: One to Fuse and Three to Keep the Nascent Fusion Pore Open. Science, 335(6074):1355-1359

9. Xiaoyang Wu, Qing-Tao Shen, Daniel S. Oristian, Catherine P. Lu, Qinsi Zheng, Hong-Wei Wang and Elaine Fuchs (2010) Skin Stem Cells Orchestrate Directional Migration by Regulating Microtubule-ACF7 Connections through GSK3β. Cell, 144(3):341-352

10. Qing-Tao Shen, Xiao-Chen Bai, Lei-Fu Chang, Yi Wu, Hong-Wei Wang, Sen-Fang Sui (2009) Bowl-shaped oligomeric structures on membranes as DegP’s new functional forms in protein quality control. Proc. Natl. Acad. Sci. USA, 106:4858-4863

11. Xiao-Yan Song, Qing-Tao Shen, Shu-Tao Xie, Xiu-Lan Chen, Cai-Yun Sun, Yu-Zhong Zhang (2006) Broad-spectrum antimicrobial activity and high stability of Trichokonins from Trichoderma koningii SMF2 against plant pathogens. FEMS Microbial Lett., 260(1):119-125

12. Chang-Zhen Liu, Li He, Qing-Tao Shen, Xiang-Hui Li, Sen-Fang Sui (2005) Leucine zipper domain is important for actin binding of p57. Progress in Biochemistry and Biophysics, 32(8):718-725

13. Qing-Tao Shen, Xiu-Lan Chen, Cai-Yun Sun, Yu-Zhong Zhang (2004) Dissecting and exploiting nonribosomal peptide synthetases. Acta Biochim Biophys Sin (Shanghai), 36(4):243-249