Zhi-Jie Liu Professor, Executive Director

InstituteiHuman Institute
Research AreaMolecular Structural Biology

1980-1984 B.Sc. in Physics, Tianjin Normal University, China
1984-1987 M.Sc. in Quantum Chemistry and Physical Chemistry, Tianjin Normal University
1987-1991 Lecturer, Tianjin Normal University
1991-1995 Ph.D. in Protein Crystallography, Institute of Biophysics, Chinese Academy of Sciences
1995-1995 Postdoctoral Fellow, Department of Crystallography, University of Pittsburgh
1995-1997 Postdoctoral Fellow, Department of Biochemistry & Molecular Biology, University of Georgia
1997-2002 Assistant Research Scientist, Department of Biochemistry & Molecular Biology, University of Georgia
2002-2005 Associate Research Scientist, Department of Biochemistry & Molecular Biology, University of Georgia
2005-2013 Professor, Institute of Biophysics, Chinese Academy of Sciences
2013 – present Professor, iHuman Institute, ShanghaiTech University
helix2Research Interests

The current research is focused on the study of human G protein coupled receptors (GPCR) and related cell signal transductions.

The long term research focus will be shifted towards GPCR mediated signaling transduction mechanism in neuron cells by integrating different imaging and functional analysis methods. 

The special interest is GPCR biased signaling which is important for developing effective and side effect free drugs.

bookSelected Publications

1.T. Hua, K. Vemuri, S. Nikas, R. Laprairie, Y. Wu, L. Qu, M. Pu, A. Korde, S. Jiang, J.H. Ho, GW. Han, D. Ding, X. Li, H. Liu, S. M. Hanson, S. Zhao*, L. Bohn*, A. Makriyannis*, R Stevens. Liu ZJ* (2017) Crystal Structure of agonist-bound Human Cannabinoid Receptor CB1. Nature, 547 (7664): 468-471.

2.Song G, Yang D, Wang Y, Graaf C.D, Zhou Q, Jiang S, Liu K, Cai X, Dai A, Lin G, Liu D, Wu F, Wu Y, Zhao S, Ye L, Han G.W, Lau J, Wu B, Hanson M.A, Liu Z.J*, Wang M.W*, and Steven R.C* (2017) Human GLP-1 receptor transmembrane domain structure in complex with allosteric modulators, Nature,546(7657):312-315.

3.T. Hua, K. Vemuri, M. Pu, L. Qu, GW. Han, Y. Wu, S. Zhao, W. Shui, S. Li, A. Korde, R. Laprairie, E. Stahl, J. Ho, N. Zvonok, H. Zhou, I. Kurareva, B. Wu, Q. Zhao, M. Hanson, L. Bohn*, A. Makriyannis*, R Stevens*. ZJ. Liu* (2016) Crystal Structure of the Human Cannabinoid Receptor CB1. Cell, 167(3):750-762.

4.Liu B, Ouyang S, Makarova K, Xia Q, Zhu Y, Li Z, Guo L, Koonin E, Liu ZJ*, Huang L*. (2015) A primase subunit essential for efficient primer synthesis by an archaeal eukaryal-type primase. Nat Commun. 6:7300.

5.J. Gu, Y. Feng, X. Feng, C. Sun, L. Lei, W. Ding, F. Niu, L. Jiao, M. Yang, Y. Li, X. Liu, J. Song, Z. Cui, D. Han, C. Du, Y. Yang, S. Ouyang*, Z. J.  Liu*, W. Han*, (2014) Structural and Biochemical Characterization Reveals LysGH15 as an Unprecedented EF-Hand-Like Calcium-Binding Phage Lysin, PLOS Pathogens, 10(5) e1004109.

6.Zhao, L., Hua, t., Crowley, C., Ru, H., Ni, X., Shaw, N., Jiao, L., Ding, W., Qu, L., Hung, L., Huang, W., Liu, L., Ye, K., OuYang, S*, Cheng, G*, and Liu, Z. J*, (2014) Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage, Cell Res, 24(3):344-58

7.Niu, F., Shaw, N., Wang, Y., Jiao, L., Ding, W., Li, X., Zhu, P., Upur, H., OuYang, S*,Cheng, G*, and Liu, Z. J*, (2013) Structure of the Leanyer Orthobunyavirus Nucleoprotein-RNA complex reveals novel architecture for RNA encapsidation, PNAS, 110(22):9054-9.

8.Ru, H., Ni, X., Zhao, L., Crowley, C., Ding, W., Hung, L., Shaw, N., Cheng, G*. and Liu, Z. J*, (2013) Structural basis for termination of AIM2-mediated signaling by p202. Cell Res, 23(6):855-8.

9.OuYang, S, Song, X, Wang, Y, Ru, H., Shaw, N., Jiang, Y., Niu, F., Zhu, Y., Qiu, W., Parvatiyar, K., Li, Y., Zhang, R., Cheng, G*., and Liu, Z. J*, (2012) Structural Analysis of the STING Adaptor Protein Reveals a Hydrophobic Dimer Interface and Mode of Cyclic di-GMP Binding, Immunity, 36(6): 1073-1086.

10.Liu, Z. J*, Chen, L., Wu, D., Ding, W., Zhang H., Zhou, W., Fu, Z. & Wang, BC*, (2011) A multi-dataset data-collection strategy produces better diffraction data. Acta Cryst A67, 544-549.

11.Wu, D., Li, Y., Song, GJ, Zhang, R., Joachimiak, A., Shaw, N., & Liu, Z. J*. (2009) Structural basis for the inhibition of human MTHFS by N10-substituted folate analogues. Cancer Research, 69(18), 7294-301. (Cover)

12.Shaw, N., Zhao, M., Cheng, C., Xu, H., Saarikettu, J., Li, Y., Da, Y., Yao, Z., Silvennoinen, O., Yang, J.*, Liu, Z. J*., Wang, B. C. & Rao, Z. (2007) The multifunctional human p100 protein 'hooks' methylated ligands. Nat Struct Mol Biol, 14(8), 779-84.

13.Shaw, N., Cheng, C., and Liu, Z. J*, (2007) Procedure for reductive methylation of protein to improve crystallizability. Nature Protocols DOI: 10.1038/nprot.2007.287

14.Liu, Z.J., Vysotski, E.S., Chen, C.J., Rose, J.P., Lee, J., and Wang, B.C.* (2000). Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure, Protein Sci 9, 2085-2093.

15.Liu, Z.J., Sun, Y.J., Rose, J., Chung, Y.J., Hsiao, C.D., Chang, W.R., Kuo, I., Perozich, J., Lindahl, R., Hempel, J., and Wang, B.C.* (1997). The first structure of an aldehyde dehydrogenase reveals novel interactions between NAD and the Rossmann fold. Nat Struct Biol, 4, 317-326.

16.Liu, Z.J., and Liang, D.C.* (1995). A Preliminary study on the determination of integral radius in molecular replacement method. Acta Biophysica Sinica, 11, 139-143.